MANEA Polyclonal Antibody

• Background:

  N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]) .[supplied by OMIM, Sep 2008]

• Description:

  This is a MANEA Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  MANEA, ENDO, hEndo

• Gene ID:

  79694

• Swiss Prot:

  Q5SRI9

• Reactivity:

  Human

• Immunogen:

  Recombinant fusion protein of human MANEA

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1mg/mL

• Dilution:

  WB 1:500-1:2000

• Buffer:

  PBS with 0.01% thiomersal,50% glycerol, pH7.3.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  25kDa/35kDa/41kDa

• Observed Molecular Weight:

  37kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG