GRP78 Polyclonal Antibody

• Background:

  GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 110), and multiple single aa substitituion. Over aa 1654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.

• Description:

  This is a GRP78 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  BiP, GRP-78, HSP70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, HSPA5

• Gene ID:

  3309

• Swiss Prot:

  P11021

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant Human GRP78 protein expressed by E.coli.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB

• Purification Method:

  Antigen affinity purification

• Assay Type:

  Antibody

• Concentration:

  1.5mg/mL

• Dilution:

  WB 1:500-1:1000

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH 7.4

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  26kDa

• Observed Molecular Weight:

  26kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG