PYCARD Polyclonal Antibody

• Background:

  Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions.

• Description:

  This is a PYCARD Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  A, Asc, TMS-, TNS1, masc, CARD5, TMS-1, 9130417A21Rik

• Gene ID:

  66824

• Swiss Prot:

  Q9EPB4

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of mouse PYCARD

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1mg/mL

• Dilution:

  WB 1:500-1:2000, IHC 1:50-1:200

• Buffer:

  PBS with 0.05% proclin300,50% glycerol, pH7.3.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  30kDa

• Observed Molecular Weight:

  45-70KDa

• Host or Source:

  Rabbit

• Isotype:

  IgG