PYCARD Polyclonal Antibody

• Background :

  Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions.

• Description :

  This is a PYCARD Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms :

  A, Asc, TMS-, TNS1, masc, CARD5, TMS-1, 9130417A21Rik

• Gene ID :

  66824

• Swiss Prot :

  Q9EPB4

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant fusion protein of mouse PYCARD

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Polyclonal Antibody

• Applications :

  WB, IHC

• Purification Method :

  Affinity purification

• Assay Type :

  Antibody

• Concentration :

  1mg/mL

• Dilution :

  WB 1:500-1:2000, IHC 1:50-1:200

• Buffer :

  PBS with 0.05% proclin300,50% glycerol, pH7.3.

• Storage Conditions :

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight :

  30kDa

• Observed Molecular Weight :

  45-70KDa

• Host or Source :

  Rabbit

• Isotype :

  IgG