Recombinant Human B-lymphocyte antigen CD20 (MS4A1) -Detergent (Active)

• Gene Name:

  MS4A1

• UniProt:

  P11836

• Expression Region:

  1-297aa

• Organism:

  Homo sapiens

• Target Sequence:

  MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

• Tag:

  C-terminal 10xHis-tagged

• Source:

  Mammalian cell

• Field of Research:

  Cancer

• Assay Type:

  MP-Detergent Transmembrane Protein & Active Protein & In Stock Protein

• Relevance:

  B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602).

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  Measured by its binding ability in a functional ELISA. Immobilized Human CD20 at 2 μg/mL on an Nickel Coated plate can bind Anti-CD20 recombinant antibody (CSB-RA015007A1HU), the EC50 is 4.034-6.968 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized Human CD20 at 2 μg/mL on an Nickel Coated plate can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC50 is 8.137-9.704 ng/mL.

• Length:

  Full Length

• Form:

  Liquid

• Buffer:

  50 mM HEPES，0.15 M NaCl， 0.05% DDM，0.01% CHS，pH7.5

• Molecular Weight:

  34.4 kDa

• References & Citations:

  "CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins." Polyak M.J., Li H., Shariat N., Deans J.P. J. Biol. Chem. 283:18545-18552 (2008)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

• CAS Number:

  9000-83-3