Anti-c-Myc tag (mouse monoclonal) (clone 9E10)

Anti-c-Myc tag (mouse monoclonal) (clone 9E10)

• Background: c-Mycis derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: c-Myc tagged fusion proteins
• Immunogen: Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt:P01106
• Clonality: Monoclonal
• Applications: Flow cytometry (Flowcyt), Immunohistochemistry (IHC), paraffin, Immunoprecipitation (IP), Western blot (WB)
• Dilution: 5-10 µg/mL (IHC) paraffin, 1-5 µg/mL (IP), 1-4 µg/mL (Flowcyt), 0.5-2 µg/mL (WB)
• Purity: Immunoglobulin purified on Protein A.
• Format: Liquid at 1 mg/mL.
• Precautions: Immunohistochemistry (paraffin sections): positive control tissue: perfused brain sections, liver, spleen.Immunoprecipitation note: this antibody will not  immunoprecipitate native c-Myc protein.Flow cytometry note: Intracellular or extracellular staining, depends upon particular expression.
• Storage Conditions: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.