Anti-BirA (mutated/TurboID)

Anti-BirA (mutated/TurboID)

• Background: Tagging a protein withTurboIDallows studying protein interactions in different types of cells, organs and developmental stages. This is a suitable tool for proximity labelling experiments as described inMairet al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: BirA (mutated/TurboID)
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Recombinant mutated BirA protein fromE.coliproduced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Western blot (WB)
• Dilution: 1 : 5000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl, of sterile water
• Molecular Weight: 35 kDa
• References & Citations: Persynet al. (2024).A Nitrogen-Specific Interactome Analysis Sheds Light on the Role of the SnRK1 and TOR Kinases in Plant Nitrogen Signaling. Mol Cell Proteomics. 100842. doi: 10.1016/j.mcpro.2024.100842.Liuet al. (2024). Activation of kappa opioid receptor suppresses post-traumatic osteoarthritis via sequestering STAT3 on the plasma membrane. Cell Commun Signal. 2024 Jun 18;22(1):335.doi: 10.1186/s12964-024-01709-4.Medicaet al. (2023). Proximity-dependent mapping of the HCMV US28 interactome identifies RhoGEF signaling as a requirement for efficient viral reactivation. PLoS Pathog. 2023 Oct 2;19(10):e1011682.doi: 10.1371/journal.ppat.1011682.Shiet al. (2023). Protocol to identify protein-protein interaction networks in Solanum tuberosum using transient TurboID-based proximity labeling. STAR Protoc. 2023 Sep 20;4(4):102577.doi: 10.1016/j.xpro.2023.102577.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.