Anti-VSR1 | Vacuolar-sorting receptor 1

• Background:

  Vacuolar-sorting receptor (VSR)involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S albumin and maybe other seed storage proteins to protein storage vacuoles (PSVs) in seeds. May also be implicated in targeting N-terminal propeptide containing proteins to lytic vacuoles. Subcellular localisation: Golgi apparatus membrane.Alternative names:  BP80-like protein b, Epidermal growth factor receptor-like protein 1, AtELP, AtELP1, Spot 3 protein

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Nicotiana tabacum

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Recombinant, His6-tagged, VSR1 fromArabidopsis thaliana,UniProt:P93026, TAIR:At3g52850

• Clonality:

  Polyclonal

• Applications:

  Immunofluorescence (IF), Western blot (WB)

• Dilution:

  1: 500 (IF), 1: 1000-1: 5000 (WB)

• Purity:

  Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

• Format:

  Liquid at 2 mg/mL.

• Molecular Weight:

  68 | 80 kDa

• Precautions:

  Higher apparent molecular weight of VSR1 is due to three determined glycosylations and several more predicted

• References & Citations:

  Fujiet al. (2016). The Adaptor Complex AP-4 Regulates Vacuolar Protein Sorting at the trans-Golgi Network by Interacting with VACUOLAR SORTING RECEPTOR1. Plant Physiol. 2016 Jan;170(1):211-9. doi: 10.1104/pp.15.00869. (Western blot, Arabidopsis thaliana)Kuniedaet al. (2013). Spatiotemporal secretion of PEROXIDASE36 is required for seed coat mucilage extrusion in Arabidopsis. Plant Cell. 2013 Apr;25(4):1355-67. doi: 10.1105/tpc.113.110072. (Western blot, Arabidopsis thaliana)Yamadaet al. (2005). Endosomal proteases facilitate the fusion of endosomes with vacuoles at the final step of the endocytotic pathway. Plant J. 2005 Mar;41(6):888-98. doi: 10.1111/j.1365-313X.2005.02349.x. (Immunofluorescence, Nicotiana tabacum)

• Storage Conditions:

  Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4