Anti-FITC | fluorescein isothiocyanate, HRP-conjugated (mono

Anti-FITC | fluorescein isothiocyanate, HRP-conjugated (mono

• Background: FITCis a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: FITC molecule
• Immunogen: Highly purified fluorescein isothiocyanate isomer 1
• Clonality: Monoclonal
• Applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB)
• Dilution: The optimum working dilution should be determined by titration by end user.1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
• Purity: Purified IgG.1 in PBS pH 7.2, HRP conjugated.
• Format: Lyophilized
• Reconstitution: For reconstitution add 0.5 ml of sterile water
• Precautions: This product has not been tested for cross-reactivity with other fluorescent compounds
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.