Anti-H3K4me3 | Histone H3, trimethylated lysine 4 (H3K4me3)

• Background:

  Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. Methylation of histone H3K4 is associated with activation of gene transcription.

• HS Code:

  38221900

• Gene ID:

  8350

• UniProt:

  P68431

• Host:

  Rabbit

• Reactivity:

  Human, Solanum lycopersicum

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated synthetic peptide

• Clonality:

  Polyclonal

• Applications:

  Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)

• Field of Research:

  Plant Epigenetics/DNA methylation

• Dilution:

  1-5 µg/IP (ChIP-seq), 1 : 10 000 (Dot), 1 : 100 (ELISA), 1 : 200 (IF), 1 : 2000 (PA), 1 : 1000 (WB)

• Purity:

  Antigen affinity purified

• Format:

  Liquid

• Additionnal Information:

  Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300 at concentration of 1.3 µg/µl.

• References & Citations:

  Song et al. (2024) . Whole-genome landscape of histone H3K4me3 modification during sperm cell lineage development in tomato. BMC Plant Biol. 2024 Jun 27;24 (1) :610. doi: 10.1186/s12870-024-05318-8. Mursalimov et al. (2019) . Cytological Techniques to Study Cytomixis in Plant Male Meiosis. Methods Mol Biol. 2020;2061:117-129. doi: 10.1007/978-1-4939-9818-0_9. Liu et al. (2018) . Transcriptomics analyses reveal the molecular roadmap and long noncoding RNA landscape of sperm cell lineage development. Plant J. 2018 Jul 26. doi: 10.1111/tpj.14041.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• Prediction Reactivity:

  Arabidopsis thaliana, Mouse, Oryza sativa, Populus sp., Zea maysSpecies of your interest not listed? Contact us

• CAS Number:

  9007-83-4