Anti-H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4)

Anti-H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4)

• Background: Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. The dimethylation of both arginine 2 (H3R2me2) and lysine 4 (H3K4me2) of H3 are both known marks to have opposing affects. R2me2 maintains transcriptional silence by silencing Set1 mediated K4 methylation, in which K4 methylation is normally associated with active chromatin. The protein arginine methyltransferase PRMT6 can methylate H3R2 in vivo, and overexpression of this enzyme downregulates Hox and Myc dependent genes, both of which are targets of H3K4 methylation.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Human
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide
• Clonality: Polyclonal
• Applications: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)
• Dilution: 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 200 (IF), 1 : 50 (IHC), 1 : 500 (WB)
• Purity: Immunogen affinity purified serum.
• Format: Liquid
• Molecular Weight: 15 kDa
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.