Goat anti-Mouse IgG2, FITC conjugated

Goat anti-Mouse IgG2, FITC conjugated

• Background: Goat anti-mouse IgG2, FITC (fluorescein-5-isothiocyanate) conjugatedis a secondary antibody which is subclass specific and binds to mouse IgG1 in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody.
• CAS Number: 9007-83-4
• Host: Goat
• Reactivity: Mouse IgG2a and IgG2b, subclass specific
• Not reactive in: It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion
• Immunogen: Purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b,
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)(frozen), Immunofluorescence (IF)
• Dilution: 1 : 20-1 :80 The optimum working dilution of each conjugate should be established by titration before being used, Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal
• Purity: Immunogen affinity purified goat IgG.
• Format: Lyophilized
• Reconstitution: For reconstitution, add 1 ml of sterile distilled water
• Storage Conditions: Stored lyophilize material at 4°C and for long time storage at -20°C. After reconstirution make aliquots and Store at -20°C to avoid repeated freeze-thaw cycles. Prior to use, thaw the aliquot slowly in the dark at ambient temperature, spin down again and prepare the working dilutions by adding sterile phosphate buffered saline (PBS, pH 7,2). Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day.