Anti-Ferritin 1-2 (plant)

Anti-Ferritin 1-2 (plant)

• Background: Ferritinforms a 24-subunit cage for storage of mineral iron. In plants, ferritin is predominantly localized in the plastids, and its expression is upregulated in response to iron or oxidative stress.
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Brassica oleracea, Hordeum vulgare, Medicago truncatula, Pisum sativum, Spinacia oleracea
• Immunogen: Purified ferritin from dried peas,Pisum sativumL. After extraction from pea flour, the ferritin was further purified by gel filtration chromatography to >95% purity as estimated from a Coomassie-stained gel.Antibody is most likely to bind to all ferritin isoforms from pea however it has not been confirmed as yet.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 5000-10 000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 23 kDa in legumes, 24 kDa (Arabidopsis thaliana)
• Precautions: Note, the calculated molecular weight of pea ferritin is 28 kDa. Removal of the N-terminal targeting sequence upon protein import into plastids results in a protein with an apparent mol weight of ~23 kDa.This antibody is also recognizing horse ferritin (above 100 ng in Western blot).
• References & Citations: Truonget al. (2024). Apo-siderophores promote growth of iron-deficient Arabidopsis plants by mobilizing iron from roots to shoots and reducing oxidative stress in roots. Plant Stress, Volume 12, June 2024, 100488.Jianget al. (2022) Reactive effects of pre-sowing magnetic field exposure on morphological characteristics and antioxidant ability of Brassica juncea in phytoextraction. Chemosphere. 2022 Sep;303(Pt 1):135046. doi: 10.1016/j.chemosphere.2022.135046. Epub 2022 May 23. PMID: 35618056.Bastowet al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Upon reconstitution in water, store the antibody in 20 % glycerol (final) at -20°C.