Anti-DYKDDDDK (binds to Sigma FLAG®, clone FG4R)

Anti-DYKDDDDK (binds to Sigma FLAG®, clone FG4R)

• Background: FLAG-tagis a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated DYKDDDDK (FLAG) synthetic peptide
• Clonality: Monoclonal
• Applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature), 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user
• Purity: Total IgG. Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
• Format: Liquid
• Storage Conditions: Store at -20°C for up to 2 years.