Anti-PPDK | Pyruvate orthophosphate dikinase

Anti-PPDK | Pyruvate orthophosphate dikinase

• Background: PPDK (Pyruvate, phosphate dikinase 1)is involved in formation of phosphoenolpyruvate and activated by light-induced dephosphorylation. Inhibited by dark-induced phosphorylation. PPDK is a low-abundance enzyme in C3 plants while it is a key enzyme of C4 photosynthesis.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana,Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays
• Not reactive in: Cucumis sativus
• Immunogen: Purified recomibinant enzyme consisting of residues 72-947 ofZea mays,UniProt:P11155Peptide used to elicit this antibody is conserved in both isoforms of PPDK in rice: PPDK1 and PPDK2.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 25 000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 200 µl of sterile water in 40% glycerol to a final protein concentration of 100 ng/µl
• Molecular Weight: 102 | 95 kDa
• Precautions: PPDK levels inr C3 plants likeArabidopsis thalianaandHordeum vulgareare very low and PPDK protein is very dilute in most tissues of C3 plants. To perform detection in C3 plants leaf proteins needs to be concentrated before western blot,Chastainet al. (2002).
• References & Citations: Shenet al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.