Anti-NRT2,1 | Nitrate transporter 2,1

Anti-NRT2,1 | Nitrate transporter 2,1

• Background: NRT2.1is a high affinity nitrate transporter which is up-regulated by nitrate. Functions as a repressor of lateral root initiation independently of nitrate uptake.  Synonymes: Protein ACH1, Protein LATERAL ROOT INITIATION 1, ACH1, ATNRT2.1, ATNRT2:1,, LIN1, NITRATE TRANSPORTER 2, NITRATE TRANSPORTER 2.1, NITRATE TRANSPORTER 2:1, NRT2, NRT2.1, NRT2:1, NRT2;1AT
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana
• Not reactive in: Alexandrium pacificum, Glycine max, Solanum tuberosum
• Immunogen: KLH-conjugated synthetic peptide derived fromArabidopsis thalianaNRT2.1 C-terminal sequence, UniProt:O82811, TAIR:AT1G08090
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 5000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 57.7 | 45 kDa
• Precautions: Microsomal fraction is recommended to be used as it will have higher amount of NRT2,1 protein, If a total protein extract is used SDS should be applied from the beginning in extraction buffer as well as protease inhibitor coctail
• References & Citations: Zouet al. (2019). Phosphorylation at Ser28 stabilizes the Arabidopsis nitrate transporter NRT2.1 in response to nitrate limitation. J Integr Plant Biol. 2019 Jul 24. doi: 10.1111/jipb.12858.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.