Goat anti-Human IgE (epsilon chain), DyLight® 800 conjugated

• Background:

  Goat anti-human IgE (ε chain), DyLight®800 Conjugatedis a secondary antibody conjugated toDyLight® 800, which binds to human IgE (ε chain) in immunological assays.DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Human IgEDyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

• Host:

  Goat

• Immunogen:

  Purified human IgE (epsilon chain)

• Clonality:

  Polyclonal

• Dilution:

  1 : 20-1 : 2000 for most applications

• Purity:

  Immunogen affinity purified goat IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

• References & Citations:

  Plundrichet al. (2019). Binding of peanut allergen Ara h 2 with Vaccinium fruit polyphenols. Food Chemistry Vol 284, 30 June 2019, Pages 287-295.

• Storage Conditions:

  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4