Donkey anti-Goat IgG (H&L), DyLight® 550 conjugated, min cross reactivity to human, mouse, rabbit or rat IgG

• Background:

  Donkey anti-Goat IgG (H&L) - DyLight® 550 Conjugated, min cross reactivity to human, mouse, rabbit or rat IgGis a secondary antibody conjugated toDyLight® 550, which binds to all goat IgGs in immunological assays.DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

• Host:

  Donkey

• Immunogen:

  Purified goat IgG, whole molecule

• Clonality:

  Polyclonal

• Dilution:

  1 : 20-1 : 2000 for most applications

• Purity:

  Immunogen affinity purified donkey IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

• Precautions:

  Based on immunoelectrophoresis, this antibody reacts with:  heavy (γ) chains on goat IgG  light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins IgG/serum from human, mouse, rabbit or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

• Storage Conditions:

  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4