Anti-N-YFP | N-terminal of YFP

Anti-N-YFP | N-terminal of YFP

• Background: YFP (Yellow Fluorescent Protein)is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: N-YFP tagged proteins fromArabidopsis thaliana, Escherichia coli, Nicotiana tabacum
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide derived from N-terminal of YFP protein.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 2000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Precautions: YFP molecular weight is 27 kDa, however detected band is going to have MW increased by a fused protein
• References & Citations: Liet al. (2021) Two ubiquitin-associated ER proteins interact with COPT copper transporters and modulate their accumulation, Plant Physiology, 2021;, kiab381, https://doi.org/10.1093/plphys/kiab381Lunget al. (2021) Oxylipin signaling in salt-stressed soybean is modulated by ligand-dependent interaction of Class II acyl-CoA-binding proteins with lipoxygenase. Plant Cell. 2021 Dec 17:koab306. doi: 10.1093/plcell/koab306. Epub ahead of print. PMID: 34919703.Schultz-Larsenet al. (2018). The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. Cell Rep. 2018 Nov 27;25(9):2329-2338.e5. doi: 10.1016/j.celrep.2018.11.011.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.