Goat anti-Rabbit IgG Fc, HRP conjugated

Goat anti-Rabbit IgG Fc, HRP conjugated

• Background: Goat anti-rabbit IgG Fc (two heavy chains with constant domains)is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) inimmunological assays.
• CAS Number: 9007-83-4
• Host: Goat
• Reactivity: Rabbit IgG Fc (two heavy chains with constant domains)
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Purified Rabbit IgG
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: The optimal working dilution should be determined by the investigator
• Purity: Immunogen affinity purified goat IgG.
• Format: Lyophilized
• Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
• Precautions: This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.
• Storage Conditions: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.