Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum

• Background:

  Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragmentis a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) inimmunological assays.

• CAS Number:

  9007-83-4

• Host:

  Goat

• Reactivity:

  Human IgG Fc (two heavy chains with constant domains)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified human IgG

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  The optimal working dilution should be determined by the investigator

• Purity:

  Immunogen affinity purified goat IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 0.55 ml of sterile water

• Precautions:

  This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunglobulins or non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins or IgG F(ab)' fragment based on immunoelectrophoresis.The antibody ahs been absorbed against solid phase normal bovine, mouse and rabbit serum in addition to mouse and rabbit IgG.

• Storage Conditions:

  Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.