Goat anti-Human IgG Fc, F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum

• Background:

  Goat anti-human IgG Fc (two heavy chains with constant domains)F(ab)'2 fragmentis a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) inimmunological assays.

• CAS Number:

  9007-83-4

• Host:

  Goat

• Reactivity:

  Human IgG Fc (two heavy chains with constant domains)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified human IgG

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

• Purity:

  Immunogen affinity purified goat IgG.

• Format:

  Liquid

• Precautions:

  This antibody reacts with the heavy (gamma) chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins, or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins or mouse and rabbit IgG based on immunoelectrophoresis.

• Storage Conditions:

  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.