Anti-FtsZ | Procaryotic cell division GTPase

Anti-FtsZ | Procaryotic cell division GTPase

• Background: FtsZ(cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Candidatus Moranella endobiaPCIT ,EscherichiaDH5a, Escherichia coliBW 25113,Shigella flexneri
• Not reactive in: Alysiella filiformis, B. subtilis,Haloferax mediterranei(Listerasp.,Neisseria meningitidis,Pseudomonas aeruginosa,Staphylococcus aureus(strain MRSA252), cyanobacteria
• Immunogen: KLH-conjugated synthetic peptide derived from known bacterial sequences of FtsZ includingE.coliUniProt:P0A9A6
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Western blot (WB)
• Dilution: 1 : 100-1 : 200 (IF), 1 : 1000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 40 | 42 kDa
• References & Citations: Chakrabortyet al. (2024). Dynamics of interdomain rotation facilitates FtsZ filament assembly. JBC, 7 May 2024, 107336.Westlundet al. (2023). Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli. Curr Genet. 2023 Jun;69(2-3):153-163. doi: 10.1007/s00294-023-01266-2. Epub 2023 Apr 6.Zhao, Zhao & Nie (2022)Nanocarriers based on bacterial membrane materials for cancer vaccine delivery. Nat Protoc. 2022 Oct;17(10):2240-2274. doi: 10.1038/s41596-022-00713-7. Epub 2022 Jul 25. PMID: 35879454.Vedyaykinet al. (2020). SulA is able to block cell division in Escherichia coli by a mechanism different from sequestration. Biochem Biophys Res Commun . DOI: 10.1016/j.bbrc.2020.03.012Ranjitet al. (2020). Chlamydial MreB Directs Cell Division and Peptidoglycan Synthesis in Escherichia coli in the Absence of FtsZ Activity. mBio. 2020 Feb 18;11(1). pii: e03222-19. doi: 10.1128/mBio.03222-19. (Immunofluorescence)Sekaret al. (2018). Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. Mol Syst Biol. 2018 Nov 5;14(11):e8623. doi: 10.15252/msb.20188623.Mücklet al. (2018). Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach. PLoS One. 2018 Sep 11;13(9):e0198058. doi: 10.1371/journal.pone.0198058. eCollection 2018.Pendeet al. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803.Söderströmet al. (2014). Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.