Goat anti-Guinea pig IgG (H&L), HRP conjugated

Goat anti-Guinea pig IgG (H&L), HRP conjugated

• Background: Goat anti-guinea pig IgGis a secondary antibody conjugated to HRP which binds to all guinea pig IgGs inimmunological assays.
• CAS Number: 9007-83-4
• Host: Goat
• Reactivity: Heavy chains on guinea pig IgG and light chains on all guinea pig immunoglobulins
• Not reactive in: Non-immunoglobulin guinea pig serum proteins
• Immunogen: Purified Guinea pig IgG, whole molecule
• Clonality: Polyclonal
• Applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
• Purity: Immunogen affinity purified goat IgG.
• Format: Lyophilized
• Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
• References & Citations: Jinet al. (2019).  YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy. Brazilian Journal of Medical and Biological Research (2019) 52(1): e7914.
• Storage Conditions: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.