Anti-Enolase 2

Anti-Enolase 2

• Background: Enolase(2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate.Alternative name: Bifunctional enolase 2/transcriptional activator
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Helianthus annuus
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: RecombinantArabidopsis thalianaenolase UniProt:P25696-1, TAIR:At2g36530
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 2000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 200 µl of sterile water
• Molecular Weight: 47.7 kDa (Arabidopsis thaliana)
• Precautions: Antibody is specific for the ENO2 isoform (cytosolic and active isoform), see data below
• References & Citations: Zhanget al. (2020). A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun. 2020 Sep 9;11(1):4509.doi: 10.1038/s41467-020-18234-w.Zhanget al. (2018). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Chenet al.(2009) System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolic regulation. Plant Physiol.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.