Donkey anti-Mouse IgG (H&L), DyLight® 488 conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG

• Background:

  Donkey anti-Mouse IgG (H&L), DyLight®488 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgGis a secondary antibody conjugated toDyLight® 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

• Host:

  Donkey

• Immunogen:

  Purified Mouse IgG, whole molecule

• Clonality:

  Polyclonal

• Dilution:

  1 : 20-1 : 2000 for most applications

• Purity:

  Immunogen affinity purified donkey IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

• References & Citations:

  Mauryaet al. (2017). Hedgehog signaling regulates ciliary localization of mouse odorant receptors. Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9386-E9394. doi: 10.1073/pnas.1708321114.

• Storage Conditions:

  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4