Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to human IgG/serum

• Background:

  Goat anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated,is a F(ab)'2 fragment of a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

• CAS Number:

  9007-83-4

• Host:

  Goat

• Clonality:

  Polyclonal

• Dilution:

  1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

• Purity:

  Immunogen affinity purified IgG, F(ab)'2 fragment.

• Format:

  Liquid

• Storage Conditions:

  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.