Anti-SE | Serrate RNA effector molecule (rabbit antibody)

• Background:

  SerrateRNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Malus domestica, Nicotiana benthamina, Nicotiana tabacum

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated synthetic peptide chosen fromArabidopsis thalianaserrate protein sequenceQ9ZVD0,At2g27100

• Clonality:

  Polyclonal

• Applications:

  Chromatin Immunoprecipitation (ChIP), Immunolocalization (IL), RNA Immunoprecipitation (RIP), Western blot (WB)

• Dilution:

  1 : 500 (IL), 1 : 1000 (WB)

• Purity:

  Immunogen affinity purified serum in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  81 | 80 kDa

• Precautions:

  Suggested blotting conditions: 8% gel, tank blotting, 200 mA/ 1h to nitrocellulose membrane

• References & Citations:

  Lianget al. (2022). Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading. Nat Commun. 2022 Mar 8;13(1):1217. doi: 10.1038/s41467-022-28872-x. PMID: 35260568; PMCID: PMC8904849.Liet al. (2023). JANUS, a spliceosome-associated protein, promotes miRNA biogenesis in Arabidopsis. Nucleic Acids Res . 2023 Nov 22:gkad1105. doi: 10.1093/nar/gkad1105.Liet al. (2021). In vitro Reconstitution Assays of Arabidopsis 20S Proteasome. Bio-protocol 11(7): e3967. DOI: 10.21769/BioProtoc.3967.Liet al. (2020). Apple SERRATE negatively mediates drought resistance by regulating MdMYB88 and MdMYB124 and microRNA biogenesis. Hortic Res. 2020 Jul 1;7:98.doi: 10.1038/s41438-020-0320-6. (ChIP)Liet al. (2019). Global co-transcriptional splicing in Arabidopsis and the correlation with splicing regulation in mature RNAs. Mol Plant. 2019 Nov 20. pii: S1674-2052(19)30367-3. doi: 10.1016/j.molp.2019.11.003.Wanget al. (2019). The PROTEIN PHOSPHATASE4 Complex Promotes Transcription and Processing of Primary microRNAs in Arabidopsis. Plant Cell. 2019 Feb;31(2):486-501. doi: 10.1105/tpc.18.00556.de Francisco Amorimet al. (2018). The U1 snRNP Subunit LUC7 Modulates Plant Development and Stress Responses via Regulation of Alternative Splicing. Plant Cell. 2018 Nov;30(11):2838-2854. doi: 10.1105/tpc.18.00244. Epub 2018 Oct 11.Kryovrysanakiet al. (2018). SERRATE, a miRNA biogenesis factor, affects viroid infection in Nicotiana benthamiana and Nicotiana tabacum. Virology. 2018 Dec 29;528:164-175. doi: 10.1016/j.virol.2018.12.011.Maet al. (2018). Arabidopsis Serrate Coordinates Histone Methyltransferases ATXR5/6 and RNA Processing Factor RDR6 to Regulate Transposon Expression. Dev Cell. 2018 Jun 18;45(6):769-784.e6. doi: 10.1016/j.devcel.2018.05.023.Wanget al. (2018). SWI2/SNF2 ATPase CHR2 remodels pri-miRNAs via Serrate to impede miRNA production. Nature. 2018 May;557(7706):516-521. doi: 10.1038/s41586-018-0135-x. Epub 2018 May 16.Liet al. (2016). Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis. PLoS Genet. 2016 Nov 21;12(11):e1006422. doi: 10.1371/journal.pgen.1006422. eCollection 2016.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4