Anti-ABA | Abscisic acid (C1) (for immunolocalization)

Anti-ABA | Abscisic acid (C1) (for immunolocalization)

• Background: Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Abscisic acid (C1) inArabidopsis thaliana,Eucalyptus globulus, Petuniahybrida L., Pinus radiata, Populus trichocarpa
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: BSA-conjugated abscisic acid (C1) via C1 carboxyl group
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Immunolocalization (IL)
• Dilution: The optimal working dilution should be determined by the investigator
• Purity: Total IgG. Protein G purified in PBS with 50 % glycerol.
• Format: Liquid
• Precautions: The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde, ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive, The antibodies will predominantly recognize only ABA and its glucosylester
• References & Citations: Wojciechowskaet al. (2020). Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa. Int J Mol Sci , 21 (6)Diniset al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007.Kovalevaet al. (2017). ABA and IAA control microsporogenesis in Petunia hybrida L. Protoplasma. 2017 Nov 13. doi: 10.1007/s00709-017-1185-x.Escandónet al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.Ondzighi-Assoumeet al. (2016). Environmental Nitrate Stimulates Root Tip Abscisic Acid Accumulation via Release from Inactive Stores. Plant Cell. 2016 Feb 17. pii: TPC2015-00946-RA.Jesuset al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56–66.Lacuestaet al. (2013). Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering. Tree Physiol. May;33(5):537-49.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.