Anti-Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1

Anti-Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1

• Background: OEP 75 or Toc75; Chloroplast outer envelope membrane protein fromPisum sativum(pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane;  YaeT; outer membrane protein assembly complex, (TIGR03303);  FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Pisum sativum,some cross-reactivity was observed for cyanobacteria including:Synechocystis, Synechococcus and Thermosynechococcus
• Not reactive in: Physcomitrella patens, Zea mays
• Immunogen: psTOC75; Predicted POTRA Domain #1; Amino acids, 158-241; Expressed and purified inE. coliusing the Impact System from NEB. Peptide confirmed by MALDI.Q43715
• Clonality: Polyclonal
• Applications: Flow cytometry (Flow cyt), Immunolocalization (IL),Western blot (WB)
• Dilution: 1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 200 µl of sterile water
• Molecular Weight: 88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
• Precautions: Antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.