Anti-Cyt c | Cytochrome c

• Background:

  Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.

• HS Code:

  38221900

• Gene ID:

  9326599

• UniProt:

  D7KMK0 , D7LY03

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Brassica oleracea, Glycine max, Pisum sativum, Zea mays

• Not reactive in:

  Arabidopsis thaliana CytC6, Chinesecuscuta sp.

• Immunogen:

  KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2), TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)

• Clonality:

  Polyclonal

• Applications:

  Immunolocalization (IL), Western blot (WB)

• Field of Research:

  Mitochondria | Respiration

• Dilution:

  1: 100 (IL), 1 : 5000 (WB)

• Purity:

  Immunogen affinity purified serum in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  12.5 | 14 kDa (for Arabidopsis thaliana)

• Precautions:

  https://www.agrisera.com/en/artiklar/goat-anti-rabbit-igg-hl-ap-conjugated-.htmlThe presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death)

• References & Citations:

  Soria et al. (2024) .Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants. Current Plant Biology Volume 37, March 2024, 100322.Canal et al. (2024) .Cytochrome c levels affect the TOR pathway to regulate growth and metabolism under energy-deficient conditions. New Phytol. 2024 Mar;241 (5) :2039-2058. Guo et al. (2021) The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in Arabidopsis thaliana, Biochemical and Biophysical Research Communications, Volume 578,2021, Pages 63-69, ISSN 0006-291X, https://doi.org/10.1016/j.bbrc.2021.09.022. (https://www.sciencedirect.com/science/article/pii/S0006291X21013164) Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Dai et al. (2020) . Pentatricopeptide repeat protein DEK46 is required for multi-sites mitochondrial RNA editing and maize seed development. J Exp Bot. 2020 Jul 25; eraa348.doi: 10.1093/jxb/eraa348. Waltz et al. (2019) . Small is big in Arabidopsis mitochondrial ribosome. Nat Plants. 2019 Jan;5 (1) :106-117. doi: 10.1038/s41477-018-0339-y.Doronina et al. (2019) . Structural and Functional Features of the Wheat Embryo Sac’s Antipodal Cells during Differentiation. Russ J Dev Biol 50, 194–208. (immunolocalization)

• Storage Conditions:

  Store lyophilized at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and Store at -80°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• Prediction Reactivity:

  Cytc1 and cytc2 from following species: A. theoprasi, Brassica napus, Brassica oleracea, Cannabis sativa, C. maxima, Chlamydomonas reinhardtii (peptide target partially conserved), Lupinus luteus, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrium patens, Ricinus communis, S. nigra, Solanum lycopersivum, Vitis vinifera.Species of your interest not listed? Contact us

• CAS Number:

  9007-83-4