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Anti-Xylose

CAT: 0451-AS07 267Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0451-AS07 267Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
Higher plants and algae
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Xylose residues bound to the N-glycan in beta 1,2
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Carbohydrates
Dilution
1 µg/ml (ELISA), 2 µg/10 ml incubation buffer (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
10-100 for various glycoproteins
Precautions
Negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.Positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250
Additionnal Information
Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.
References & Citations
Xu et al. (2023) . The Golgi-localized transporter OsPML4 contributes to manganese homeostasis in rice. Plant Sci. 2023 Dec 2:339:111935.doi: 10.1016/j.plantsci.2023.111935. Ropitaux et al. (2023) . Subcellular localization of core beta (1, 2) -xylosylated N-glycoproteins in the green microalgae Chlamydomonas reinhardtii. Algal Research Volume 77, January 2024, 103366.Xavier et al. (2021) Inactivation of N-Acetylglucosaminyltransferase I and a1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans. Frontiers in Plant Science. Volume 12. doi: 10.3389/fpls.2021.634023Yang et al. (2021) . Golgi-localized manganese transporter PML3 regulates Arabidopsis growth through modulating Golgi glycosylation and cell wall biosynthesis. New Phytol. 2021 Jan 17. doi: 10.1111/nph.17209. Epub ahead of print. PMID: 33454966.Lucas et al. (2019) . Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2019 Nov 27. doi: 10.1111/tpj.14620. Lucas et al. (2019) . Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2019 Nov 27. doi: 10.1111/tpj.14620.Hou et al. (2019) . Identification and characterization of a novel glycoprotein core xylosidase from the bacterium Elizabethkingia meningoseptica. Biochemical and Biophysical Research Communications Available online 27 July 2019.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Higher plants
Product Datasheet
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