Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase

Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase

• Background: PRKribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate.
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Synechocystis sp. PCC6803, Synechococcus PCC 7942, Thalassiosira pseudonana, Oryza sativa, Zea mays
• Not reactive in: Proteobacteria
• Immunogen: KLH-conjugated peptide derived from known PRK sequences includingArabidopsis thalianaUniProt:P25697, TAIR:At1g32060
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 44 | 39 kDa (A. thaliana)
• Precautions: Antibody detects PRK using a load from 4-20 µg/well of a chloroplast fraction, incubation over night at 4°C
• References & Citations: Fukayamaet al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9.Pérez-Ruizet al. (2017). NTRC-dependent redox balance of 2-Cys peroxiredoxins is needed for optimal function of the photosynthetic apparatus. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12069-12074. doi: 10.1073/pnas.1706003114.Raiet al. (2017). Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep. 2017 Apr 5;7:45732. doi: 10.1038/srep45732. (microalga, western blot)Nikkanenet al. (2016). Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. Plant Cell Environ. 2016 Aug;39(8):1691-705. doi: 10.1111/pce.12718.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.