Anti-HSP70 | Salmonid heat shock protein 70, Affinity purified

• Background:

  Heat-shock protein 70(Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

• Host:

  Rabbit

• Reactivity:

  Oncorhynchus mykiss (Rainbow trout),Danio rerio(Zebrafish)

• Not reactive in:

  Gasterosteus aculeatus

• Immunogen:

  KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 UniProt:B5X4Z3.

• Clonality:

  Polyclonal

• Applications:

  Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

• Dilution:

  5 µg of antibodies in reaction mixture (IP), 1 : 100 (IHC), 1 : 15 000 (WB)

• Purity:

  Immunogen affinity purified serum in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 200 µl of sterile water

• Molecular Weight:

  70 kDa

• Precautions:

  This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive

• References & Citations:

  Mottolaet al. (2020). Comp Biochem Physiol A Mol Integr Physiol. 2020 Feb;240:110629. doi: 10.1016/j.cbpa.2019.110629.Gallantel al. (2017). Physiological responses to a short-term, environmentally realistic, acute heat stress in Atlantic salmon, Salmo salar. FACETS.Lewiset al. (2016). Different Relationship between hsp70 mRNA and hsp70 Levels in the Heat Shock Response of Two Salmonids with Dissimilar Temperature Preference. Front Physiol. 2016 Nov 7;7:511. eCollection 2016.Curieet al. (2008). β‐Adrenergic Stimulation Enhances the Heat‐Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4