Phospho-Catenin beta (Tyr654) Polyclonal Antibody

• Background:

  Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.

• Description:

  This is a Phospho-Catenin beta (Tyr654) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  Beta catenin, Beta-catenin, Cadherin associated protein, Catenin (cadherin associated protein), beta 1,88kDa, Catenin beta 1, Catenin beta-1, CATNB, CHBCAT, CTNB1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP00000165222, OTTHUMP0000016522

• Swiss Prot:

  P35222

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from human Catenin-β around the phosphorylation site of Tyr654

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC-p, ELISA

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:40000

• Buffer:

  PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  85 kDa

• Observed Molecular Weight:

  75 kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG