PRKDC Polyclonal Antibody

• Background:

  Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D) J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V (D) J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) . The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.

• Description:

  This is a PRKDC Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  DNA dependent protein kinase catalytic subunit, DNA PK catalytic subunit, DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNAPK, DNAPK catalytic subunit, DNPK 1, DNPK1, Hyper radiosensitivity of murine scid mutation, complementin

• Swiss Prot:

  P78527

• Reactivity:

  Human, Mouse

• Immunogen:

  Synthesized peptide derived from the C-terminal region of human DNA-PKCS

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC-p, IF, ELISA

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000

• Buffer:

  PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  469 kDa

• Observed Molecular Weight:

  450 kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG