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RBFOX3 Polyclonal Antibody

CAT: 0866-RD70467A-01Size: 60 μLDry Ice: NoHazardous: No
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CAT#:0866-RD70467A-01Size:60 μL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.
Description
This is a RBFOX3 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
Synonyms
FLJ56884, FLJ58356, Fox-1 homolog C, fox1 homolog C, Fox3, FOX3NeuN, hexaribonucleotide binding protein 3, HRNBP3, NEUN, neuronal nuclei, Rbfox3, RFOX3, RNA binding protein fox-1 homolog 3, RNA binding protein, fox 1 homolog (C. elegans) 3
Swiss Prot
Q8BIF2
Reactivity
Mouse, Rat
Immunogen
KLH conjugated Synthetic peptide corresponding to NeuN
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Polyclonal Antibody
Applications
WB, IHC
Purification Method
Affinity purification
Assay Type
Antibody
Concentration
1 mg/mL
Dilution
WB 1:500-1:2000, IHC 1:300-1:800
Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
46-48 kDa
Observed Molecular Weight
46-48 kDa
Host or Source
Rabbit
Isotype
IgG

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