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PCNA Polyclonal Antibody

CAT: 0866-RD70222A-01Size: 60 μLDry Ice: NoHazardous: No
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CAT#:0866-RD70222A-01Size:60 μL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.
Description
This is a PCNA Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
Synonyms
ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, Pcna/cyclin, PCNA, PCNAR, Polymerase delta accessory protein, Proliferating cell nu
Swiss Prot
P12004, P17918, P04961
Accession Number
BC020766
Reactivity
Human, Mouse, Rat
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse PCNA
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Polyclonal Antibody
Applications
WB, IHC
Purification Method
Affinity purification
Assay Type
Antibody
Concentration
270 μg/mL
Dilution
WB 1:1000-1:3000, IHC 1:300-1:800
Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
33 kDa
Observed Molecular Weight
33 kDa
Host or Source
Rabbit
Isotype
IgG

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