EIF5B Polyclonal Antibody

• Background:

  Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3) . Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

• Description:

  This is a EIF5B Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  EIF5B, IF2

• Gene ID:

  9669

• Swiss Prot:

  O60841

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human EIF5B (NP_056988.3) .

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB, IF

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000 IF 1:50-1:200

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  138 kDa

• Observed Molecular Weight:

  179 kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG