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Beta actin Polyclonal Antibody

CAT: 0866-RD240338A-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0866-RD240338A-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle) . Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Description
This is a beta actin Control Antibody from Reddot Biotech. This product is for Research Use Only.
Synonyms
ACTB, Actin beta, Actx, Actin cytoplasmic 1, b actin, A26C1A
Gene ID
57935
Swiss Prot
Q7ZVF9
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant Zebrafish Actin, cytoplasmic 2 protein
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Control Antibody
Applications
WB, IHC
Purification Method
Antigen Affinity Purification
Assay Type
Antibody
Concentration
0.85 mg/mL
Dilution
WB 1:1000-1:2000 IHC 1:100-1:300
Buffer
PBS with 0.05% Proclin300, 50% glycerol, pH7.3
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
41 kDa
Observed Molecular Weight
41 kDa
Host or Source
Rabbit
Isotype
IgG

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