GAPDH Polyclonal Antibody

• Background:

  Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

• Description:

  This is a GAPDH Control Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  Aging associated gene 9 protein, BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS, p38 component

• Gene ID:

  317743

• Swiss Prot:

  Q5XJ10

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Control Antibody

• Applications:

  WB, IHC

• Purification Method:

  Antigen Affinity Purification

• Assay Type:

  Antibody

• Concentration:

  0.66 mg/mL

• Dilution:

  WB 1:3000-1:6000 IHC 1:300-1:500

• Buffer:

  PBS with 0.05% Proclin300, 50% glycerol, pH7.3

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  35 kDa

• Observed Molecular Weight:

  35 kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG