EGFR Polyclonal Antibody

• Background :

  Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action.

• Description :

  This is a EGFR Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms :

  Avian erythroblastic leukemia viral (v erb b) oncogene homolog, Cell growth inhibiting protein 40, Cell proliferation inducing protein 61, EGF R, EGFR, EGFR, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), Epide

• Swiss Prot :

  P00533

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthesized peptide derived from human EGFR around the non-phosphorylation site of Tyr1016.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Polyclonal Antibody

• Applications :

  WB, IHC-p, IF, ELISA

• Purification Method :

  Affinity purification

• Assay Type :

  Antibody

• Concentration :

  1 mg/mL

• Dilution :

  WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000

• Buffer :

  PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

• Storage Conditions :

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight :

  134 kDa

• Observed Molecular Weight :

  134 kDa

• Host or Source :

  Rabbit

• Isotype :

  IgG