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Human PKLR Recombinant Protein

CAT: 0519-PROTP30613-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0519-PROTP30613-01Size:10 µg
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Description
Human PKLR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 47-574aa. Application (s) : Enzyme Activity, SDS-PAGE.
Synonyms
Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK
Gene Name
PKLR
UniProt
P30613
Tag
His-Tag
Source
E. coli
Sequence
[object Object]
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Cancer
Purification
> 90% by SDS-PAGE
Concentration
1mg/ml (determined by Bradford assay)
Bioactivity
Specific activity is > 80 units/mg. One unit will convert 1.0 umole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37°C.
Form
Liquid
Molecular Weight
59.2 kDa (549aa) confirmed by MALDI-TOF
Shipping Conditions
Available
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

UniProtKB · P30613

Pyruvate kinase PKLR

KPYR_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P30613
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PKLR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.1.40
Processing
—
Secondary accessions
O75758, P11973
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

Allosteric enzymeKinaseTransferase

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMagnesiumManganeseMetal-bindingNucleotide-bindingPotassiumPyruvate

Disease

Disease variantHereditary hemolytic anemia

Biological process

Glycolysis

PTM

Phosphoprotein

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