MIP-1a Macrophage Inflammatory Protein-1 Alpha Rat Recombinant Protein (CCL3)

• Description:

  Macrophage Inflammatory Protein-1 alpha Rat Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton. The MIP-1a is purified by proprietary chromatographic techniques.

• Synonyms:

  Small inducible cytokine A3; CCL3; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; G0S19-1 protein; SIS-beta; PAT 464.1; chemokine (C-C motif) ligand 3; MIP1A; SCYA3; G0S19-1; LD78ALPHA

• Gene Name:

  CCL3

• UniProt:

  P50229

• Reactivity:

  Rat

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 97.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

• Bioactivity:

  The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc. of 60 ng/ml corresponding to a Specific Activity of 16,667IU/mg.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized MIP-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

• Other Gene Names:

  C-C motif chemokine 3

• Subcellular Location:

  Secreted.