IGFBP-4 Insulin-Like Growth Factor Binding Protein-4 Human

• Description:

  IGFBP-4 Human Recombinant amino acids Asp22- Glu258, produced in HEK293 cells and fused with a polyhistidine tag at the C-terminus. IGFBP4 predicted Mw is 27kDa and on SDS-PAGE appears as a 32kDa band under denaturing conditions. IGFBP4 is purified by proprietary chromatographic techniques.

• Synonyms:

  Insulin-like growth factor-binding protein 4; IBP-4; IGF-binding protein 4; IGFBP-4; IGFBP4; IBP4; BP-4; HT29-IGFBP

• Gene Name:

  IGFBP4

• UniProt:

  P22692

• Reactivity:

  Human

• Source:

  HEK293

• Sequence:

  [object Object]

• Purification:

  Greater than 95.0% as determined by SDS-PAGE.

• Bioactivity:

  The ED50 range is 0.01-0.09µg/ml as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized IGFBP4 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Other Gene Names:

  Insulin-like growth factor-binding protein 4

• Subcellular Location:

  Secreted.