GLU-C S.aureus Glutamyl endopeptidase Staphylococcal Recombinant Protein

• Description:

  Recombinant Staphylococcal GLU-C produced in E.coli is a single, non-glycosylated polypeptide chain containing a total of 267 amino acids and having a molecular mass of 28.9kDa.

• Synonyms:

  Glutamyl endopeptidase (EC:3.4.21.19) ; Endoproteinase Glu-C; Staphylococcal serine proteinase; V8 protease; V8 proteinase; sspA

• Gene Name:

  SSPA

• UniProt:

  P0C1U8

• Reactivity:

  Staphylococcus aureus

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 95% as determined by SDS-PAGE.

• Form:

  Sterile Filtered lyophilized powder.

• Reconstitution:

  2

• Function:

  Preferentially cleaves peptide bonds on the carboxyl- terminal side of aspartate and glutamate. Along with other extracellular proteases it is involved in colonization and infection of human tissues. Required for proteolytic maturation of thiol protease SspB and inactivation of SspC, an inhibitor of SspB. It is the most important protease for degradation of fibronectin-binding protein (FnBP) and surface protein A, which are involved in adherence to host cells. May also protect bacteria against host defense mechanism by cleaving the immunoglobulin classes IgG, IgA and IgM. May be involved in the stability of secreted lipases.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized GLU-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GLU-C should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Other Gene Names:

  Glutamyl endopeptidase

• Subcellular Location:

  Secreted