Exodus-2 Human Recombinant Protein (CCL21)

• Description:

  Exodus-2 Human Recombinant produced in E. coli as a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12219 Dalton. The CCL21 is purified by proprietary chromatographic techniques.

• Synonyms:

  Small inducible cytokine A21; CCL21; Beta chemokine exodus-2; 6Ckine; Secondary lymphoid-tissue chemokine; SLC; chemokine (C-C motif) ligand 21; ECL; CKb9; TCA4; SCYA21; MGC34555

• Gene Name:

  CCL21

• UniProt:

  O00585

• Reactivity:

  Human

• Tissue Specificity:

  Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 97.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

• Bioactivity:

  Determined by its ability to chemoattract human total lymphocyte population using a concentration range of 10-100ng/ml.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized Exodus2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized Exodus-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

• Other Gene Names:

  C-C motif chemokine 21

• Subcellular Location:

  Secreted.