TRIM33 Tripartite Motif Containing 33 Human Recombinant Protein

• Description:

  TRIM33 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 124,041 Dalton. TRIM33 is expressed with a -10xHis tag at N-terminus and purified by proprietary chromatographic techniques.

• Synonyms:

  E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33; TRIM33; KIAA1113; RFG7; TIF1G; ECTO; PTC7; TF1G; TIFGAMMA; TIF1GAMMA

• Gene Name:

  TRIM33

• UniProt:

  Q9UPN9

• Reactivity:

  Human

• Tissue Specificity:

  Expressed in stem cells at the bottom of the crypts of the colon (at protein level) . Expressed in colon adenomas and adenocarcinomas (at protein level) . Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.

• Source:

  Sf9 insect cells

• Purification:

  Greater than 80.0% as determined by SDS-PAGE.

• Form:

  Sterile Filtered clear solution.

• Function:

  Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity) . Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade) .

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

• Other Gene Names:

  E3 ubiquitin-protein ligase TRIM33

• Subcellular Location:

  Nucleus