M-CSF Macrophage Colony Stimulating Factor Rat Recombinant Protein HEK

• Description:

  MCSF Rat Recombinant produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254) . M-CSF is disulfide-linked homodimer containing 2 x 222 a.a chains.

• Synonyms:

  CSF-1; Lanimostim; MCSF; MGC31930; M-CSF

• Gene Name:

  CSF1

• UniProt:

  Q8JZQ0

• Reactivity:

  Rat

• Source:

  HEK293

• Purification:

  Greater than 95.0% as determined by SDS-PAGE.

• Bioactivity:

  The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the MCSF in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance (By similarity) .

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Other Gene Names:

  Macrophage colony-stimulating factor 1

• Subcellular Location:

  Cell membrane