HP-NAP Neutrophil-activating protein A Helicobacter Pylori Recombinant Protein

• Description:

  HP-NAP Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain (Met1-Ala144) containing 154 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 18.2kDa.

• Synonyms:

  DNA protection during starvation protein; Bacterioferritin; HP-NAP; Neutrophil-activating protein A; NAP A; dps; napA

• Gene Name:

  DPS

• UniProt:

  P43313

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 95.0% as determined by SDS-PAGE.

• Form:

  Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  Prepare about 0.5mg/ml working stock solution with deionized water and let the lyophilized pellet dissolve completely. HP-NAP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

• Function:

  Protects DNA from oxidative damage by sequestering intracellular Fe (2+) ion and storing it in the form of Fe (3+) oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe (2+) ions, which prevents hydroxyl radical production by the Fenton reaction (By similarity) . Required for the survival in the presence of oxidative stress. Dps is also a virulence factor that activates neutrophils, mast cells and monocytes. It binds to neutrophil-glycosphingolipids and to sulfated carbohydrates on mucin. It might have a role in the accumulation of neutrophils and monocytes at the site of infection. Induces superoxide anion generation, adhesion and chemotaxis of neutrophils, through a pertussis toxin-sensitive pathway involving MAP kinases.

• Shipping Conditions:

  Available

• Storage Conditions:

  Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

• Other Gene Names:

  DNA protection during starvation protein

• Subcellular Location:

  Cytoplasm.