ENPP1 Ectonucleotide Pyrophosphatase Human Recombinant Protein

• Description:

  ENPP1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 98-925) containing a total of 840 amino acids, having a molecular mass of 96.5kDa (calculated) though it migrates at approximately 110kDa on SDS PAGE, the ENPP1 is also composed of a 2 a.a N-terminal linker, a 4 a.a C-terminal linker and fused to a 6 a.a His tag at C-Terminus. The Human ENPP1 is purified by proprietary chromatographic techniques.

• Synonyms:

  Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Membrane component chromosome 6 surface marker 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1; ENPP1; M6S1; NPPS; PC1; PDNP1; NPP1; PC-1; PCA1; ARHR2; COLED

• Gene Name:

  ENPP1

• UniProt:

  P22413

• Reactivity:

  Human

• Tissue Specificity:

  Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis.

• Source:

  HEK293

• Sequence:

  [object Object]

• Purification:

  Greater than 95.0% as determined by SDS-PAGE.

• Form:

  Filtered lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

• Function:

  By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity and function.

• Shipping Conditions:

  Available

• Storage Conditions:

  Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.

• Other Gene Names:

  Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

• Subcellular Location:

  Cell membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Secreted